The expression of surface markers in the intestinal tissues was measured in a separate study . The samples include mononuclear cells isolated from the small intestine and jejunum. The cell-cell interactions in mononuclear cells are being investigated with flow cytometry. This study confirmed that SIV-mac was able to infect ileum and colonic mononuclear cells, resulting in cell activation as seen by CD38 up regulation. ART administration did not further enhance the SIV-induced cell activation.
proliferation of cells in the follicle-associated lymphoid tissue (A). Representative photomicrographs are shown for CD3 negative B cells (control, B), T cells (C), follicular dendritic cells (FDC), and macrophages (M). The proliferating cells (black nuclei) are shown with calcein violet. A is the lymph node, B the white pulp, C the T cell zone, and D is an SIV infected lymphoid tissue (Rule lymph node). The distribution of CD3 negative cells (B, C, and D) is similar in the three histological sections, suggesting that the LN is intact. The majority of proliferating cells were within the B cell follicles and were negative for T cells, indicating that the B cells of the follicle-associated lymphoid tissue are proliferating. The numbers of proliferating cells in the follicles (A and B) or in the interfollicular region (C and D) were not significantly different in SIV and HIV-infected LN. Similarly, the numbers of proliferating CD3 negative cells in the interfollicular region (C) and compartment (D) of SIV-infected LN were not significantly different from those of HIV-infected LN in the same animals. These comparisons were performed with Student's t test. The SIV-infected lymph node appeared to be intact.
Histological assessment of the SIV infected intestines showed that the changes in ileum were minimal, and included large accumulations of mononuclear cells present in the mucosa, especially in the Peyer patches. In the lamina propria, there was some thickening of the intestinal mucosa. d2c66b5586