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The respiratory system is the primary physiological site of stress resistance in aquatic vertebrates. In this study we tested the hypothesis that the respiratory system of the freshwater turtle Trachemys scripta is capable of mounting an effective stress response to a single stressor (e.g., thermal stress) and to repeated stresses. Specifically, we tested the hypotheses that (1) the stress response is observable when there is a decrease in ventilation and/or (2) there is a loss of water and Na(+) in the lung tissue. This information would increase our understanding of the acute thermal stress response of the respiratory system in a non-mammalian vertebrate, and would broaden our understanding of the mechanisms involved in the acute stress response of vertebrates generally. The turtles were euthanized and the lungs excised. The lungs were dissected and processed for X-ray microanalysis and histopathology. Turtles exposed to elevated temperatures for 1 h had evidence of increased permeability and microvascular congestion, i.e. histopathological changes, in the lung tissue, while differences in the ventilatory system were not apparent. In response to single and repeated (12 h) stress exposure there was a loss of water in the lung, Na(+) in the air spaces and Na(+) in the tissue. We also found evidence of increases in permeability and microvascular congestion in the lung parenchyma in turtles exposed to multiple stressors. Our findings indicate that the turtles could mount an effective stress response to acute stressors, and these data are consistent with the hypothesis that increased permeability and microvascular congestion are components of the airway, pulmonary and systemic stress response.Directions
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