H9 GFP, ZFXOver1 and ZFXOver2 cell lines were then cultured for 2-3 weeks in N2B27 medium on a defined medium containing 10% ES-grade FBS and mESC. In the three cell lines, ZFX was expressed in N2B27 cultures (Figure 2B). Immunostaining for SOX2 and OCT4 revealed the undifferentiated ES cell state of these cultures (data not shown). YFP was undetectable in the BAC transgenic clones (Figure 2C). We also compared the expression of ZFX in H9 ZFXOver2 cells to several other hESC lines by Western blot analysis (Figure 2D). ZFX levels were similar to those in H9 ZFXOver2 cells with other hESCs. ZFX expression from the BAC transgene did not affect the expression of OCT4 and NANOG in H9 ZFXOver2 cells (Figure 2E). However, we previously observed that forced ZFX expression did induce upregulation of SOX2 in mES cells . ZFX expression did not alter SOX2 expression in H9 ZFXOver2 cells (Figure 2F). After ZFX induction, the expression of the hESC markers was not significantly altered in the H9 ZFXOver2 cells (Figure 2G).
The MATLAB environment is based on the MathWorks MATLAB® Compiler, or MMC, which is a proprietary language developed by MathWorks to facilitate the building of applications. MMC understands the MATLAB language and various task APIs and provides efficient intermediate language compilers for compiling functions and scripts.
The MATLAB® Engine is the core of the MATLAB product. It compiles, executes, and interprets MATLAB scripts and programs, as well as runs the M-files and M-links created by the MMC. It also allows the exchange of data between MATLAB applications, the MathWorks M-files and M-links, and other programming languages, including C, C++, and Visual Basic. 827ec27edc